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Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice

  • Kelsey R. Pflepsen
  • Cristina D. Peterson
  • Kelley F. Kitto
  • Lucy Vulchanova
  • George L. Wilcox
  • Carolyn A. FairbanksEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1937)

Abstract

Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.

Key words

Intrathecal Gene therapy AAV gene therapy Intrathecal gene delivery Direct lumbar puncture 

Notes

Acknowledgments

This work was supported by NIDA grant R01DA035931-01, DoD grant W81XWH-15-1-0494, T32 training grant T32DA07234 to K.P., and T32 training grant T32DA007097 to C.P.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kelsey R. Pflepsen
    • 1
  • Cristina D. Peterson
    • 2
  • Kelley F. Kitto
    • 2
  • Lucy Vulchanova
    • 2
  • George L. Wilcox
    • 2
    • 3
    • 4
  • Carolyn A. Fairbanks
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of PharmaceuticsUniversity of MinnesotaMinneapolisUSA
  2. 2.Department of NeuroscienceUniversity of MinnesotaMinneapolisUSA
  3. 3.Department of PharmacologyUniversity of MinnesotaMinneapolisUSA
  4. 4.Department of DermatologyUniversity of MinnesotaMinneapolisUSA

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