Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse

  • Kevin Isgrig
  • Wade W. ChienEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1937)


Inner ear gene therapy offers great potential as a treatment for hearing loss and dizziness. The surgical method used to deliver gene therapy into the inner ear is a critical step in determining the success of inner ear gene therapy. Here we describe two commonly used surgical methods for gene delivery in neonatal mouse inner ear: the round window approach and the posterior semicircular canal approach. Both of these approaches are effective at delivering gene therapy to the neonatal mouse inner ear.

Key words

Gene therapy Inner ear Posterior semicircular canal Round window Hearing loss Dizziness 



This work was supported by funds from the NIDCD Division of Intramural Research/NIH (DC000082-02 to W.W.C., as well as DC000081 to advanced imaging core). We are grateful for the NIDCD animal facility staff for caring for our animals. We would like to thank Dr. Lisa Cunningham and Dr. Nicole Schmitt for critiquing the manuscript.

Disclosures: The authors have no conflict of interest to disclose.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Institute on Deafness and Other Communication Disorders/National Institutes of HealthBethesdaUSA
  2. 2.Department of Otolaryngology-Head and Neck SurgeryJohns Hopkins School of MedicineBaltimoreUSA

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