Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples
The presence of Legionella spp. in natural and man-made water systems is a great public health concern and heavily depends on the presence of free-living amoebae. Taking advantage of the development and affordability of next-generation sequencing technology, we present here a method to characterize the whole bacterial community directly from water samples, as well as from isolated free-living amoebae.
Key wordsTargeted metagenomics High-throughput sequencing 16S rRNA Bacteria Free-living amoeba
This work was supported by the 2015–2020 State-Region Planning Contracts (CPER), European Regional Development Fund (FEDER), intramural funds from the Centre National de la Recherche Scientifique and the University of Poitiers, and Eau de Paris.
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