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Legionella pp 421-428 | Cite as

Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples

  • Vincent Delafont
  • Yoann Perrin
  • Didier Bouchon
  • Laurent Moulin
  • Yann HéchardEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1921)

Abstract

The presence of Legionella spp. in natural and man-made water systems is a great public health concern and heavily depends on the presence of free-living amoebae. Taking advantage of the development and affordability of next-generation sequencing technology, we present here a method to characterize the whole bacterial community directly from water samples, as well as from isolated free-living amoebae.

Key words

Targeted metagenomics High-throughput sequencing 16S rRNA Bacteria Free-living amoeba 

Notes

Acknowledgment

This work was supported by the 2015–2020 State-Region Planning Contracts (CPER), European Regional Development Fund (FEDER), intramural funds from the Centre National de la Recherche Scientifique and the University of Poitiers, and Eau de Paris.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vincent Delafont
    • 1
  • Yoann Perrin
    • 1
    • 2
  • Didier Bouchon
    • 1
  • Laurent Moulin
    • 2
  • Yann Héchard
    • 1
    Email author
  1. 1.Laboratoire Ecologie et Biologie des Interactions, Equipes “Microbiologie de l’Eau” et “Ecologie, Evolution, Symbiose”Université de Poitiers, UMR CNRS 7267PoitiersFrance
  2. 2.Eau de Paris, Direction de la Recherche et du Développement pour la Qualité de l’Eau, R&D BiologieIvry sur SeineFrance

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