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Legionella pp 399-417 | Cite as

The Mouse as a Model for Pulmonary Legionella Infection

  • Garrett Z. Ng
  • Anastasia Solomatina
  • Ian R. van Driel
  • Elizabeth L. HartlandEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1921)

Abstract

Infection of C57BL/6 mice with wild-type Legionella pneumophila typically results in very mild disease. However, in mice where the cytosolic recognition of flagellin is impaired by mutation, L. pneumophila infection results in more severe lung inflammation that is reminiscent of Legionnaires’ disease. This can be replicated in wild-type mice by using aflagellated mutants of L. pneumophila. These models greatly facilitate the investigation of L. pneumophila virulence factors and the complex pulmonary immune system that is triggered by infection. Here we describe methods for infecting C57BL/6 mice with aflagellated L. pneumophila, the quantification of bacterial load in the lungs and isolation and analysis of invading immune cells. These assays enable the identification of phagocyte subsets and can determine whether phagocytic cells act as a replicative niche for L. pneumophila replication.

Key words

Pneumonia Legionella Phagocytes Flow cytometry Mucosal immunology Bacterial infection 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Garrett Z. Ng
    • 1
  • Anastasia Solomatina
    • 2
    • 3
  • Ian R. van Driel
    • 1
  • Elizabeth L. Hartland
    • 4
    • 5
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyBio21 Molecular Science and Biotechnology Institute, The University of MelbourneParkvilleAustralia
  2. 2.Department of Microbiology and ImmunologyPeter Doherty Institute for Infection and Immunity, The University of MelbourneParkvilleAustralia
  3. 3.Molecular Immunology and Cell BiologyLife and Medical Sciences Institute, University of BonnBonnGermany
  4. 4.Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical ResearchClaytonAustralia
  5. 5.Department of Molecular and Translational ScienceMonash UniversityClaytonAustralia

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