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Legionella pp 21-44 | Cite as

The Pathometabolism of Legionella Studied by Isotopologue Profiling

  • Klaus HeunerEmail author
  • Mareike Kunze
  • Fan Chen
  • Wolfgang EisenreichEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1921)

Abstract

Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general 13C-labelled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.

Key words

Mass spectroscopy NMR spectroscopy Isotopologue profiling 13C isotopologue Metabolism Legionella 

Notes

Acknowledgments

We thank our coworkers Nadine Gillmaier, Vroni Herrmann, Eva Eylert, Claudia Huber, Erika Kutzner, Kerstin Rydzewski, and Eva Schunder for their enthusiastic help in establishing and optimizing the methods described in this article. This work was financed by grants from the Deutsche Forschungsgemeinschaft DFG (Bonn, Germany) (EI 384/11-1 and HE 2845/6-1, /9-1, respectively).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Robert Koch-Institut, ZBS 2, Working Group “Cellular Interactions of Bacterial Pathogens”BerlinGermany
  2. 2.Lehrstuhl für BiochemieTechnische Universität MünchenGarchingGermany

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