Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant

  • Xiaofeng GuEmail author
  • Zhe Liang
Part of the Methods in Molecular Biology book series (MIMB, volume 1933)


Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

Key words

RNA methylation 5-Methylcytosine m5m5C-RIP-seq RNA immunoprecipitation Plant 



This work was supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0101001) to X.G., National Natural Science Foundation of China (31671670) to X.G., and Recruitment program of Global Youth Expert of China to X.G.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Biotechnology Research Institute, Chinese Academy of Agricultural SciencesBeijingChina
  2. 2.Department of Biological SciencesNational University of SingaporeSingaporeSingapore

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