Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant
Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.
Key wordsRNA methylation 5-Methylcytosine m5C m5C-RIP-seq RNA immunoprecipitation Plant
This work was supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0101001) to X.G., National Natural Science Foundation of China (31671670) to X.G., and Recruitment program of Global Youth Expert of China to X.G.