Detection of Histone Modifications Associated with miRNAs
The posttranslational modifications of histones and miRNAs are key epigenetic mechanisms participating in the development, growth, and reproduction of plants. Recently, coordination between these two mechanisms has been demonstrated; each mechanism can be controlled by the other for the regulation of several biological processes. For example, the acetylation of histone H3, a key modification for chromatin remodeling and gene activation, has been linked to the actions of miRNA. In this work, we describe a method for the isolation and immunodetection of two posttranslational modifications in the residues of lysine 9 and 27 of H3, which have been associated with long miRNAs in plants.
Key wordsEpigenetics Histone modification MicroRNAs Agave angustifolia
This work was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACYT) to CD (CB2016-285898 and FC2016-1515) and a CONACYT scholarship to SHC (271240).
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