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Mimicry Technology: A Versatile Tool for Small RNA Suppression

  • Luis Manuel Villar-Martin
  • Ignacio Rubio-SomozaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1932)

Abstract

A decade ago the discovery of the target mimicry regulatory process on the activity of a mature microRNA (miRNA) enabled for the first time the customized attenuation of miRNA activity in plants. That powerful technology was named MIMIC and was based on engineering the IPS1 long noncoding transcript to become complementary to the miRNA under study. In order to avoid IPS1 degradation, the predicted miRNA-mediated cleavage site was interrupted by three additional nucleotides giving rise to the so-called MIMIC decoy. Since then, MIMIC technology has been used in several plant species and in basic and translational research. We here provide a detailed guide to produce custom-designed MIMIC decoys to facilitate the study of sRNA functions in plants.

Key words

Target mimicry miRNA Small RNA attenuation MIMIC 

Notes

Acknowledgments

The work in the MoRE group is funded by Spanish Ministry of Economy and Competitiveness (I.R-S is supported by BFU-2014-58361-JIN, RYC-2015-19154; L. V-M is supported by BES-2016-076986) and through the “Severo Ochoa Programme for Centres of Excellence in R&D” 2016-2019 (SEV-2015-0533) and the CERCA program from the Generalitat de Catalunya.

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Luis Manuel Villar-Martin
    • 1
  • Ignacio Rubio-Somoza
    • 1
    Email author
  1. 1.Molecular Reprogramming and Evolution (MoRE) Lab, Centre for Research in Agricultural Genomics (CRAG)Carrer Vall Moronta Edifici CRAGBarcelonaSpain

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