Detection of miRNAs by Tissue Printing and Dot Blot Hybridization
Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.
Key wordsmicroRNAs Tissue printing Dot blot Nylon membrane Hybridization miRNA detection
We thank the Mexican National Council of Science and Technology (CONACyT) for a fellowship to MMN. This work was financed by the CONACyT grant CB-2013-221522, and SIP grants 20170477 and 20180545. Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739 and FC-2015-2/1061.
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