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Detection of miRNAs by Tissue Printing and Dot Blot Hybridization

  • Marcelino Martínez Núñez
  • Stefan de Folter
  • Flor de Fátima Rosas-CárdenasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1932)

Abstract

Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.

Key words

microRNAs Tissue printing Dot blot Nylon membrane Hybridization miRNA detection 

Notes

Acknowledgments

We thank the Mexican National Council of Science and Technology (CONACyT) for a fellowship to MMN. This work was financed by the CONACyT grant CB-2013-221522, and SIP grants 20170477 and 20180545. Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739 and FC-2015-2/1061.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Marcelino Martínez Núñez
    • 1
  • Stefan de Folter
    • 2
  • Flor de Fátima Rosas-Cárdenas
    • 1
    Email author
  1. 1.Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional (CIBA-IPN), Ex-Hacienda San Juan MolinoTlaxcalaMexico
  2. 2.Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)IrapuatoMexico

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