Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells
Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).
Key wordsSuper-resolution microscopy 3D-SIM Immunostaining
This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Ministry of Education Singapore under its Singapore Ministry of Education Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004) to N.K.V. 3D-SIM platform (DeltaVision OMX v4 Blaze microscope) and Institute of Medical Biology (IMB) Microscopy Unit, now renamed to the A*STAR Microscopy Platform within the Skin Research Institute of Singapore (SRIS), was funded by A*STAR, Singapore.
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