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A Laboratory Model to Study T-Cell Motility

  • Navin Kumar Verma
  • Shyam Mohan Palapetta
  • Seow Theng Ong
  • Mobashar Hussain Urf Turabe Fazil
  • Madhavi Latha Somaraju Chalasani
  • Praseetha Prasannan
  • Atish Kizhakeyil
  • Dermot Kelleher
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1930)

Abstract

Regulated migration of T-lymphocytes through high endothelial venules and secondary lymphoid organs is necessary for an adaptive immune response. Uncontrolled trafficking of T-cells is implicated in many pathological conditions, including autoimmune disorders, such as psoriasis and inflammatory bowel disease. T-cell migration is regulated mainly by the αLβ2 integrin receptor LFA-1, which interacts primarily with its cognate ligand ICAM-1 expressed on the endothelium. This interaction triggers a plethora of downstream signaling pathways, which are not fully understood. Thus, in order to dissect the signal transduction processes at molecular levels and phenotypic changes in migrating T-cells, a laboratory model mimicking T-cell motility is important. Here, we describe a simple and highly reproducible in vitro model to study T-cell migration.

Key words

T-lymphocytes T-cell migration LFA-1 crosslinking ICAM-1 

Notes

Acknowledgments

This work was supported by the Lee Kong Chian School of Medicine (LKCMedicine), Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004) grants. A.K. acknowledges Ph.D. fellowship provided by LKCMedicine, Nanyang Technological University Singapore.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Navin Kumar Verma
    • 5
  • Shyam Mohan Palapetta
    • 1
    • 2
  • Seow Theng Ong
    • 1
  • Mobashar Hussain Urf Turabe Fazil
    • 1
  • Madhavi Latha Somaraju Chalasani
    • 4
    • 1
  • Praseetha Prasannan
    • 1
  • Atish Kizhakeyil
    • 1
  • Dermot Kelleher
    • 1
    • 3
  1. 1.Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  2. 2.Nanyang Institute of Technology in Health and Medicine, Interdisciplinary Graduate SchoolNanyang Technological University SingaporeSingaporeSingapore
  3. 3.Departments of Medicine and Biochemistry and Molecular BiologyUniversity of British ColumbiaVancouverCanada
  4. 4.Autoimmunity and Inflammation ProgramHospital for Special SurgeryNYUSA
  5. 5.Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore

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