An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility
The inherent ability of T-cells to migrate is critical for a fully functional immune system, both in normal immune surveillance and for mounting an adaptive immune response. At the same time, inappropriate trafficking of T-cells can be a pathological factor for immune-mediated or chronic inflammatory diseases. T-cell motility is critically dependent on a series of ligand–receptor interactions, a precisely regulated intracellular signaling, an involvement of adaptor proteins, and dynamic remodeling of the cytoskeletal systems. The leukocyte integrin LFA-1 receptor present on T-cells binds to the ligand intercellular adhesion molecule 1 (ICAM-1) and this LFA-1/ICAM-1 contact acts as a trigger for T-cell motility. In this book, we present a collection of methods and protocols that are frequently used by researchers to better understand T-cell motility in health and diseases.
Key wordsT-cell contact Integrin Signal transduction Inflammation Autoimmunity
This work was supported by the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its Singapore MOE Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004) to N.K.V.
- 19.Vasiliev JM, Gelfand IM, Domnina LV, Ivanova OY, Komm SG, Olshevskaja LV (1970) Effect of colcemid on the locomotory behaviour of fibroblasts. J Embryol Exp Morpholog 24:625–640Google Scholar
- 28.Ong ST, Chalasani MLS, Fazil MHUT, Praseetha P, Kizhakeyil A, Wright GD, Kelleher D, Verma NK (2018) Centrosome- and Golgi-localized protein kinase N-associated protein serves as a docking platform for protein kinase A signaling and microtubule nucleation in migrating T cells. Front Immunol 9:1–16CrossRefGoogle Scholar
- 30.Verma NK, Dempsey E, Conroy J, Olwell P, Mcelligot AM, Davis AM, Kelleher D, Butini S, Campiani G, Williams DC, Zisterer DM, Lawler M, Volkov Y (2008) A new microtubule targeting compound PBOX-15 inhibits T-cell migration via post-translational modification of tubulin. J Mol Med 86:457–469CrossRefGoogle Scholar
- 33.Li D, Rebecca P, Cruz MA, Molldrem JJ, Champlin RE, Ma Q (2015) Intermediate filament (IF) protein vimentin regulates T cell mediated immune response in Gvhd. Blood 126:3073Google Scholar
- 43.Molloy ES, Calabrese LH (2009) Therapy: targeted but not trouble-free: efalizumab and PML. Nat Rev Rheumatol 5418:419Google Scholar
- 44.Carson KR, Focosi D, Major EO, Petrini M, Richey EA, West DP, Bennett CL (2009) Monoclonal antibody-associated progressive multifocal leucoencephalopathy in patients treated with rituximab, natalizumab, and efalizumab: a Review from the Research on Adverse Drug Events and Reports (RADAR) Project. Lancet Oncol 10:816–824CrossRefGoogle Scholar