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S100A8/A9 in Myocardial Infarction

  • Gopalkrishna Sreejit
  • Sunil Kiran Nooti
  • Baskaran Athmanathan
  • Prabhakara Reddy NagareddyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1929)

Abstract

S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabetes, cardiovascular disease, autoimmune disorders, etc. We present here methods to quantify S100A8/A9 expression in various tissues in mouse models of myocardial infarction (MI) using flow cytometry (FC), immunofluorescence, quantitative real-time polymerase chain reaction (q-RT-PCR), and enzyme-linked immunosorbent assays (ELISA).

Key words

S100A8/A9 Myocardial infarction Neutrophils Mononuclear cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Gopalkrishna Sreejit
    • 1
  • Sunil Kiran Nooti
    • 1
  • Baskaran Athmanathan
    • 1
  • Prabhakara Reddy Nagareddy
    • 1
    Email author
  1. 1.Department of PathologyUniversity of AlabamaBirminghamUSA

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