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Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies

  • Anand Kumar SharmaEmail author
  • Radhika Khandelwal
  • Yogendra Sharma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1929)

Abstract

Secretagogin (SCGN) has recently gained attention due to its modulatory effect on insulin/CRH secretion and function. However, a large pool of speculated SCGN functions remains unexplored. A major deficiency is the lack of knowledge about the biological functions of extracellular SCGN. We here describe convenient methods for the scalable production of His-tagged and untagged mouse SCGN. The protocol is optimized to remove endotoxins, and thus the protein is suited for biological applications such as cell culture treatment or animal injections. We also outline expedient methods to check the purity of SCGN preparation for biological applications.

Key words

Secretagogin Protein purification Calcium sensor protein His-tag purification Chromatography Circular dichroism 

Notes

Acknowledgments

This work was supported partially by CSIR and DBT to YS. AKS was supported by the Senior Research Fellowship, University Grant Commission, and RK is the recipient of CSIR-GATE fellowship. Authors acknowledge occasional help from HC Amrutha during the preparation of this manuscript. Anand Kumar Sharma and Radhika Khandelwal contributed equally to this work.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Anand Kumar Sharma
    • 1
    Email author
  • Radhika Khandelwal
    • 1
    • 2
  • Yogendra Sharma
    • 1
    • 2
  1. 1.CSIR-Centre for Cellular and Molecular BiologyHyderabadIndia
  2. 2.Academy of Scientific and Innovative Research (AcSIR)GhaziabadIndia

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