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Hyperpolarized MRI for Studying Tumor Metabolism

  • Mikko I. KettunenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1928)

Abstract

Hyperpolarized magnetic resonance imaging (MRI) can be used to detect real-time in vivo tumor metabolism. Dissolution dynamic nuclear polarization method increases polarization of 13C-labeled molecules, typically [1-13C]pyruvate, which can be injected into an animal during MRI scanning. Increased polarization leads to a higher observed signal, which allows for the detection and imaging of the transfer of 13C-label between the injected marker molecule, pyruvate, and its metabolic products, most importantly lactate. This information can be used to assess the metabolic status of the tumor, for example, during therapy. Here, the basic methodology and data analysis for a preclinical hyperpolarized pyruvate experiment are described.

Key words

Hyperpolarization Pyruvate MR spectroscopy Modeling 

Notes

Acknowledgments

This work was supported by the Academy of Finland grants no. 286895 and no. 314551. The author shares dDNP-related intellectual property with GE Healthcare.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.A.I. Virtanen Institute for Molecular SciencesUniversity of Eastern FinlandKuopioFinland

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