Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics

  • Roslyn M. Bill
  • Annegret Ulke-Lemée
  • Stephanie P. Cartwright
  • Rena Far
  • Jay Kim
  • Justin A. MacDonaldEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1923)


New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

Key words

Multiple reaction monitoring Mass spectrometry MRM-MS Yeast Recombinant protein production ZIPK DAPK3 



This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Roslyn M. Bill
    • 1
  • Annegret Ulke-Lemée
    • 2
  • Stephanie P. Cartwright
    • 1
  • Rena Far
    • 2
  • Jay Kim
    • 4
  • Justin A. MacDonald
    • 3
    Email author
  1. 1.School of Life and Health SciencesAston UniversityBirminghamUK
  2. 2.Department of Biochemistry and Molecular BiologyCumming School of Medicine, University of CalgaryCalgaryCanada
  3. 3.Department of Biochemistry and Molecular Biology, Cumming School of MedicineUniversity of CalgaryCalgaryCanada
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of CalgaryCalgaryCanada

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