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High-Throughput Screening and Selection of Pichia pastoris Strains

  • Roland WeisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1923)

Abstract

Clone screening procedures for Pichia pastoris expression strain comparison rely on the availability of a cultivation environment that ensures equal growth and production capabilities for all assessed transformants. As clonal variation in such experiments is caused by diverging numbers and possibly also genomic locations of integrated (linearized) expression constructs, the productivity assessment of a larger number of strains is mandatory for selecting a set of strains for follow-up bioreactor cultivations in order to define the best-producing clone. Microscale cultivation provides the means to reliably compare growth and productivity of a large number of transformants and by that narrows down the amount of selected strains for scaling up.

Key words

Small-scale cultivation Deep-well plates High-throughput Protein production Screening 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.VALIDOGEN (formerly VTU Technology) GmbHRaaba-GrambachAustria

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