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Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha

  • Yoo Su Jin 
  • Moon Hye Yun 
  • Kang Hyun Ah Email author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1923)

Abstract

The thermotolerant methylotrophic yeast Hansenula polymorpha has been used as a host for the high-level production of recombinant proteins from industrial enzymes to therapeutic proteins. Despite favorable characteristics of the H. polymorpha-based platform for application to heterologous gene expression, several problems and limitations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. Here, H. polymorpha genetic tools and host strains, developed for authentic processing and modification of secretory recombinant proteins, are introduced with the analytical protocols.

Key words

Hansenula polymorpha Protein expression Multiple integration Secretion Glycosylation Surface display 

Notes

Acknowledgment

The work was supported by the grant No. NRF-2013M3A6-A8073554 (Global Frontier Program for the Intelligent Synthetic Biology) from the National Research Foundation of Korea (NRF).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yoo Su Jin 
    • 1
  • Moon Hye Yun 
    • 1
  • Kang Hyun Ah 
    • 1
    Email author
  1. 1.Department of Life Science, College of Natural ScienceChung-Ang UniversitySeoulRepublic of Korea

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