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Quantification of Recombinant Products in Yeast

  • Karola Vorauer-UhlEmail author
  • Gabriele Lhota
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1923)

Abstract

Quantification of various proteins expressed in yeast can be performed by different methods. In this respect, classical as well as advanced techniques can be applied, where the analysis of crude supernatants is of special interest in screening but also manufacturing.

The following chapter addresses the analytical background of the introduced methods followed by specific recommendations for the quantification of different products of industrial interest. The method portfolio includes electrophoresis, chromatography, and ELISA as classical techniques, but also biosensor-based, microfluidic and automated, miniaturized methods are introduced. Furthermore, individual strengths and perceived limitations are summarized.

Although prominent examples are described, it should be noted that individual modifications are required according to host and cultivation mode.

Key words

Protein quantification Electrophoresis HPLC ELISA Bio-layer interferometry Microfluidics CD technology Surface plasmon resonance Electrochemiluminescence assays Microchip-based assays 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyUniversity of Natural Resources and Life Sciences Vienna (BOKU)ViennaAustria

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