Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification
The harvest of a bench scale high cell density fermentation of Pichia pastoris, using standard laboratory equipment, to process products from supernatant is described. The process consists of a centrifugation step and a depth filtration, followed by a (sterile) membrane filtration. The procedure yields supernatant ready for the next purification step such as chromatography.
Key wordsCentrifugation Depth filtration
The authors would like to acknowledge Robin Ott, Karl Metzger, and Frederik Hoppe for the execution of some of the experiments outlined in this chapter and Nico Lingg for critical discussion. This work has been supported by the Austrian Federal Ministry of Science, Research and Economy (BMWFW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency (SFG), the Standortagentur Tirol, the Government of Lower Austria, and ZIT—Technology Agency of the City of Vienna through the Austrian FFG-COMET-Funding Program.
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