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Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris

  • Maria Zahid
  • Ursula RinasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1923)

Abstract

Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.

Key words

HBsAg Pichia pastoris Vaccine Virus Virus-like particle 

Notes

Acknowledgments

We are grateful to C. Gurramkonda for critical comments.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Technical Chemistry—Life Science, Leibniz University of HannoverHannoverGermany
  2. 2.Department of BiologyLahore University of Management SciencesLahorePakistan
  3. 3.Helmholtz Centre for Infection ResearchBraunschweigGermany

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