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Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells

  • Krithika HariharanEmail author
  • Petra Reinke
  • Andreas KurtzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Human pluripotent stem cells (hPSCs) have been well known for their ability to generate kidney cell types. We developed a protocol that utilizes a set of growth factors to give rise to kidney progenitors, which when differentiated further in a monolayer gives rise to podocyte precursors, mesangial cells, proximal and distal tubular epithelial cells, and collecting duct cells. This article describes in detail how to obtain each of these segment-specific kidney cell types from hPSCs. Once obtained as a homogenous population, these cells are invaluable for nephrotoxicity testing, for disease modeling, and in tissue engineering approaches such as 3D bioprinting and seeding on acellular matrices and scaffolds.

Key words

Kidney Progenitors iPS Pluripotent stem cells (PSCs) Directed differentiation Nephrotoxicity Kidney development Tubular epithelial cells Podocytes Tissue engineering 

Notes

Acknowledgments

This work was supported by Berlin-Brandenburg School for Regenerative Therapies and the German Federal Ministry of Education and Research (VIP, FKZ 03V0396, and FKZ 031A303B).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Berlin–Brandenburg Center for Regenerative Therapies (BCRT)Charité - Universitätsmedizin BerlinBerlinGermany
  2. 2.Department of Nephrology and Intensive CareCharité - University Medicine BerlinBerlinGermany

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