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Long-Term Culture of Nephron Progenitor Cells Ex Vivo

  • Aaron C. Brown
  • Ashwani K. Gupta
  • Leif OxburghEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Nephrons differentiate from the cap mesenchyme of the fetal kidney. Nephron progenitor cells that populate the cap mesenchyme efficiently balance self-renewal and epithelial differentiation to enable repeated rounds of nephron formation during development. Here we describe a method to isolate and propagate these cells from the embryonic mouse kidney. Using this method, nephron progenitor cells from a single litter of mice can be propagated to hundreds of millions of cells that express appropriate markers of the undifferentiated state and retain epithelial differentiation capacity in vitro.

Key words

Kidney Organogenesis Primary cell culture Stem cells 

Notes

Acknowledgments

Research reported in this publication is supported by the National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases under awards R01DK078161 (L.O.) and R24DK106743 (L.O.). Core facilities support was provided by the Maine Medical Center Research Institute core facilities for Molecular Phenotyping and Progenitor Cell Analysis [supported by the National Institute of General Medical Sciences (NIGMS) 5P30 GM106391]. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Aaron C. Brown
    • 1
  • Ashwani K. Gupta
    • 1
  • Leif Oxburgh
    • 1
    Email author
  1. 1.Center for Molecular MedicineMaine Medical Center Research InstituteScarboroughUSA

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