Experimental Tubulogenesis Induction Model in the Mouse

  • Aleksandra Rak-RaszewskaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)


Kidney development and induction of tubulogenesis have been studied for almost seven decades. The experimental setup of metanephric mesenchyme induction ex vivo allows to control the environment, to perform cellular manipulations, and to learn about renal development. Since the establishment of the ex vivo kidney culture technique in 1953, the method was modified to suit well the progress in biological and medical fields and still today present many advantages over the traditional in vivo studies.

Key words

Metanephric mesenchyme induction Tubulogenesis Nephrogenesis Ureteric bud Spinal cord BIO LiCl 



I would like to thank Sanna Junttila and Paula Haipus for producing the movies from which Figs. 1 and 2 were made.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Biochemistry and Molecular MedicineUniversity of OuluOuluFinland

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