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Decellularizing and Recellularizing Adult Mouse Kidneys

  • Theresa Chow
  • Jennifer Whiteley
  • Ian M. RogersEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Decellularization is the process by which resident cells are lysed and cellular debris is removed from the tissue, leaving behind the extracellular matrix scaffold. The extracellular matrix scaffold can be used for three-dimensional culturing of cells. Here we describe methods of decellularizing whole and thick sections of mouse kidneys using a 0.1% sodium dodecyl sulfate (SDS) detergent solution and strategies to repopulate whole and thick sections of decellularized mouse kidneys with cells.

Key words

Mouse kidney dissection Cannulation and perfusion Kidney decellularization Extracellular matrix Kidney recellularization Bioreactor Three-dimensional (3D) tissue culture 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Theresa Chow
    • 1
    • 2
  • Jennifer Whiteley
    • 1
  • Ian M. Rogers
    • 1
    • 2
    • 3
    Email author
  1. 1.Lunenfeld-Tanenbaum Research InstituteMt. Sinai HospitalTorontoCanada
  2. 2.Department of PhysiologyUniversity of TorontoTorontoCanada
  3. 3.Department of Obstetrics and GynecologyUniversity of TorontoTorontoCanada

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