Gene Editing in 3D Cultured Nephron Progenitor Cell Lines
During kidney development, Six2+ nephron progenitor cells (NPCs) generate nephrons, the functional units of the kidney. Isolating and expanding NPCs in vitro have enabled wide applications in both basic and translational research. Here we describe the methods to derive NPC lines from mouse embryonic kidneys in a 3D culture format as well as gene editing in the NPC lines.
Key wordsNephron progenitor cell (NPC) 3D culture NPSR culture medium Gene editing CRISPR-Cas9
This work was supported by Fundacion Dr. Pedro Guillen, Universidad Catolica San Antonio de Murcia, G. Harold and Leila Y. Mathers Charitable Foundation, The Leona M. and Harry B. Helmsley Charitable Trust, The Moxie Foundation, and National Institute of Health (R21 AG055938).