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Virus as Renal Functional Genetic Tools

  • Abhishek SharmaEmail author
  • Sandhanakrishnan Cattavarayane
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Viral vectors enable efficient transfection of ectopic DNA into hard to transfect cells. Viral vectors are normally used to obtain permanent modification of target cells, and tissues expect for the cases where integrase-deficient viruses are used. Here we describe a method to stably transfect metanephric mesenchyme cells isolated from the murine embryonic kidney at day E11.5. Using this method, it is possible to transfect hard to transfect cells and successfully evade host tissue immune response. Due to these advantages, this method has become one of the most frequently used in generating stable cell line, manipulation of tissues, and gene therapy.

Key words

Viral vectors Gene transfer Lentivirus Metanephric mesenchyme 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Abhishek Sharma
    • 1
    Email author
  • Sandhanakrishnan Cattavarayane
    • 2
  1. 1.Faculty of Biochemistry and Molecular Medicine, Biocenter OuluUniversity of OuluOuluFinland
  2. 2.Faculty of Veterinary Medicine, Section of Pathology, Department of Veterinary BiosciencesUniversity of HelsinkiHelsinkiFinland

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