Protocol to Generate Ureteric Bud Structures from Human iPS Cells
The generation of ureteric bud (UB), which is the renal progenitor that gives rise to renal collecting ducts and lower urinary tract, from human-induced pluripotent stem cells (hiPSCs) provides a cell source for studying the development of UB and kidney disease. Here we describe a stepwise and efficient two-dimensional differentiation method of hiPSCs into Wolffian duct (WD) cells. We also describe how to generate three-dimensional WD epithelial structures that can differentiate into UB-like structures.
Key wordsHuman pluripotent stem cells Anterior primitive streak Anterior intermediate mesoderm Wolffian duct Ureteric bud
We thank Dr. Peter Karagiannis, CiRA, Kyoto University, for critically reading and revising the manuscript. This study was partially supported by Otsuka Pharmaceutical Co., Ltd.; by the Japan Society for the Promotion of Science (JSPS) through its “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)” to K.O., Grant-in-Aid for Scientific Research (C) (JSPS KAKENHI Grant Number 24591195) to K.O., and Grant-in-Aid for Young Scientists (B) (JSPS KAKENHI Grant Number 17K15546) to S.I.M.; and by the Japan Agency for Medical Research and Development (AMED) through its research grant “Projects for Technological Development and Core Center for iPS Cell Research, Research Center Network for Realization of Regenerative Medicine,” and “Practical Research Project for Rare/Intractable Diseases.”