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Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum

  • Lucas Borges-Pereira
  • Célia R. S. GarciaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1925)

Abstract

Studying Ca2+ dynamics in protozoan parasites is not an easy task. Loading of parasites with commonly used Ca2+ fluorescent dyes (such as Fuo4-AM) remains as the major protocol to measure the Ca2+ oscillations inside the cell. In this chapter, we describe an alternative method to study Ca2+ signaling in Plasmodium falciparum parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca2+ assays.

Key words

Malaria Plasmodium Ca2 + Signaling Transgenic parasites 

Notes

Acknowledgments

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), process 2011/51295-5 to C.R.S.G and 2016/14411-0 to L.B.P.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical and Toxicological Analyses—School of Pharmaceutical SciencesUniversity of São PauloSão PauloBrazil

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