Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum
Studying Ca2+ dynamics in protozoan parasites is not an easy task. Loading of parasites with commonly used Ca2+ fluorescent dyes (such as Fuo4-AM) remains as the major protocol to measure the Ca2+ oscillations inside the cell. In this chapter, we describe an alternative method to study Ca2+ signaling in Plasmodium falciparum parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca2+ assays.
Key wordsMalaria Plasmodium Ca2 + Signaling Transgenic parasites
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), process 2011/51295-5 to C.R.S.G and 2016/14411-0 to L.B.P.
- 7.Borges-Pereira L, Campos BR et al (2014) The GCaMP3—a GFP-based calcium sensor for imaging calcium dynamics in the human malaria parasite Plasmodium falciparum. Methods 1:151–154Google Scholar