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Phototropism pp 215-221 | Cite as

How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening

  • Haruko Ueda
  • Ikuko Hara-NishimuraEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

Although plant organ segments bend in response to environmental stimuli such as gravity and light, they stop bending and subsequently straighten during the course of tropic responses. The straightening phenomenon can clearly be observed by setting the bent organs under microgravity and dark conditions. It has recently become clear that the straightening mechanism requires the activity of the actin-myosin XI cytoskeleton. A clinostat device makes it possible to simulate microgravity conditions by counteracting the Earth’s unilateral gravitational pull. Here, we describe a method for assessing the straightening ability of organs by clinostat analysis using Arabidopsis thaliana inflorescence stems of actin and myosin xi mutants as examples.

Key words

Arabidopsis thaliana Actin Clinostat Inflorescence stem Myosin XI Straightening 

Notes

Acknowledgments

This work was supported by the Grants-in-Aid for Scientific Research to I.H-N. (no. 15H05776) and to H.U. (nos. 15KT0151 and 16K07397) from the Japan Society for the Promotion of Science (JSPS).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science and EngineeringKonan UniversityKobeJapan

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