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Phototropism pp 207-214 | Cite as

Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station

  • Joshua P. Vandenbrink
  • John Z. KissEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

Utilization of orbiting spacecraft allows for studying biological processes in conditions of microgravity. Centrifuges on board these platforms also allow for the creation of partial gravity vectors (to simulate the Moon or Mars levels of gravity) as well as onboard 1-g controls for the space experiments. Thus, the mechanisms of gravity and light perception in plants can be analyzed in a unique environment which can give insights into fundamental processes in biology. Here, we describe the methods for preparation of a plant biology experiment with seedlings of Arabidopsis thaliana utilizing the European Modular Cultivation System (EMCS) on the International Space Station (ISS). The procedures outlined in this paper have been successfully used in several of our recent spaceflight experiments, which have given unique insights into the basic mechanisms of tropisms.

Key words

Arabidopsis thaliana European Modular Cultivation System (EMCS) Gravitropism Phototropism Plant space biology Spaceflight experiment Tropisms 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiologyUniversity of North Carolina-GreensboroGreensboroUSA

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