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Phototropism pp 165-172 | Cite as

Immunolocalization of IAA Using an Anti-IAA-C-Antibody Raised Against Carboxyl-Linked IAA

  • Takeshi NishimuraEmail author
  • Tomokazu Koshiba
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

Plant hormone indole-3-acetic acid (IAA) plays a crucial role in plant physiological events such as plant development, differentiation, and environmental responses. IAA is synthesized in specific focal cells and/or tissues such as the coleoptile tip in maize and the root tip and young leaf primordia in Arabidopsis thaliana. Recent studies have shown that formation of an IAA maxima or concentration gradient, created by the changing expression and cellular localization of IAA transport proteins, crucially controls plant physiological events. For this reason, visualization of IAA molecules at the cell and tissue levels is necessary to accurately determine the distribution of IAA in plants. Immunolocalization of IAA is a means to directly visualize IAA and observe its localization and distribution in plant cells and tissues. Here, we introduce an immunolocalization protocol to observe IAA distribution that uses a specific anti-IAA-C-antibody raised against carboxyl-linked IAA. This method is applicable for various plant samples and is reliable for specifically detecting IAA in plant tissues.

Key words

Arabidopsis 1-Ethyl-3-(3-dimethyl-aminopropyl)-carbodiimide hydrochloride (EDAC) Indole-3-acetic acid (IAA) IAA-C-antibody IAA-N1-antibody Maize 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Department of Biological SciencesGraduate School of Science, Tokyo Metropolitan UniversityHachiojiJapan

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