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Phototropism pp 157-163 | Cite as

Determination of Auxin Flow During Phototropic Responses Using Fluorescent Auxin Analogs

  • Tatsuya SakaiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

Altered auxin distribution patterns and the formation of a lateral auxin gradient are often discussed in the context of phototropism. The DR5rev::GFP auxin reporter gene is commonly used in phototropism research in Arabidopsis. This study describes a fluorescent auxin analog, NBD-NAA, as an additional tool for the determination of auxin flow during phototropic responses.

Key words

Arabidopsis Auxin gradient Fluorescent auxin analog NBD-NAA Phototropism 

Notes

Acknowledgment

This work was supported by the Japan Society for the Promotion of Science (JSPS: KAKENHI 16H01231).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Science and TechnologyNiigata UniversityNiigataJapan

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