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Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

  • Ana Paula Azambuja
  • Marcos Simoes-CostaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1920)

Abstract

The chick embryo is a powerful model for experimental embryology due to its accessibility, sturdiness, and ease of manipulation. Here we describe protocols for analysis of protein-DNA and protein-protein interactions in tissues and cells isolated from the developing chick. These assays are aimed at the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, can be used for the delineation of gene regulatory circuits.

Key words

Chick embryo Protein-DNA interactions Protein-protein interactions Chromatin precipitation (ChIP) Co-immunoprecipitation (Co-IP) 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and GeneticsCornell UniversityIthacaUSA

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