Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts
Generation of calcium signal in the cytoplasm of fertilized or parthenogenetically activated eggs has been extensively studied in the intact eggs of several biological species. Calcium transient was found to elicit a plethora of biochemical and cellular events in these cells. Remarkably, intracellular calcium signaling can also be reconstituted in cell-free environment. In this chapter, we describe the methods that allow reconstitution, detection, and quantification of the calcium signal in cell-free extracts of Xenopus oocytes and eggs.
Key wordsCalcium Signal transduction Xenopus laevis Egg Oocyte Cell-free extracts
This work was supported by the Collaboration Research grant 281027 from Kobe University to A.A.T. and the Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan 15K07083 to K-I.S.
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