Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos
The earliest steps of animal development depend upon posttranscriptional events that drive the embryonic cell cycle and guide cell fate decisions. The analysis of post-transcriptional regulatory events has relied upon the use of chimeric reporter mRNAs that encode firefly luciferase fused to potential regulatory sequences. A new and more sensitive luciferase developed recently called NanoLuc has the potential to improve reporter studies and provide new insights into the regulation of embryonic processes. Here I describe how to create and analyze reporter mRNAs encoding NanoLuc luciferase using extracts from microinjected Xenopus embryos.
Key wordsXenopus embryos mRNA microinjection NanoLuc Luciferase
Work in the Sheets lab was supported by NIH grants (R21HD076828, R01HD091921).