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Germ Cell Transplantation in Avian Species

  • Young Hyun Park
  • Young Min Kim
  • Jae Yong HanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1920)

Abstract

Germ cell transplantation technology has played a critical role in germline modification and preservation of genetic resources. Several germ cell transplantation systems have been developed, including sperm, oocyte, or germline stem cell transplantation systems in mammals. Meanwhile, in avian species, this has mostly relied on primordial germ cell (PGC) transplantation for efficient germline transmission. In this chapter, we describe how to isolate PGCs from avian embryos and produce germline chimeras through transplantation of donor PGCs to recipient embryos.

Key words

Aves Germline chimera Primordial germ cells Transplantation 

Notes

Acknowledgment

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (No. 2015R1A3A2033826).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Young Hyun Park
    • 1
  • Young Min Kim
    • 1
  • Jae Yong Han
    • 1
    Email author
  1. 1.Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life SciencesSeoul National UniversitySeoulKorea (Republic of)

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