Visualizing the Balbiani Body in Zebrafish Oocytes
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
Key wordsOocyte Balbiani body Mitochondria RNA and protein localization Primary oocytes Buckyball Immunohistochemistry Microscopy Histology
This work was partially supported by the National Institutes of Health R01GM089979.
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