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Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

  • Edwina McGlinn
  • Miriam A. Holzman
  • Jennifer H. MansfieldEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1920)

Abstract

Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

Key words

Mouse embryogenesis In situ hybridization Immunohistochemistry ß-Galactosidase Alkaline phosphatase Green fluorescent protein 

Notes

Acknowledgments

We are deeply grateful to Connie Cepko, Cliff Tabin, Carol Wicking, and to past and present members of the Cepko and Tabin labs, who developed most of the protocols as they are described here, and who have generously shared them with us. We thank Lucie Jeannotte and Kim Landry-Truchon for advice and improvements to immunohistochemistry methods. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. This work was supported by NH&MRC grants APP1051792 and APP1063967 to E.M.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Edwina McGlinn
    • 1
  • Miriam A. Holzman
    • 2
  • Jennifer H. Mansfield
    • 2
    Email author
  1. 1.EMBL Australia, Australian Regenerative Medicine InstituteMonash UniversityClaytonAustralia
  2. 2.Department of Biology, Barnard CollegeColumbia UniversityNew YorkUSA

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