Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.
Mouse embryogenesis In situ hybridization Immunohistochemistry ß-Galactosidase Alkaline phosphatase Green fluorescent protein
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We are deeply grateful to Connie Cepko, Cliff Tabin, Carol Wicking, and to past and present members of the Cepko and Tabin labs, who developed most of the protocols as they are described here, and who have generously shared them with us. We thank Lucie Jeannotte and Kim Landry-Truchon for advice and improvements to immunohistochemistry methods. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. This work was supported by NH&MRC grants APP1051792 and APP1063967 to E.M.
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