Method for Stimulation of Hippocampal Neurogenesis by Transient Microneedle Insertion

  • Shijie SongEmail author
  • Xiaoyung Kong
  • Juan Sanchez-RamosEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)


Brief stereotaxic insertion and removal of a microneedle into the hippocampus of mice result in stimulation of hippocampal neurogenesis. This approach has been previously applied to a mouse model of Alzheimer’s disease (Song et al., Cell Transplant 25:1853–1861, 2016). Further studies of fundamental cellular mechanisms of the brain’s response to micro-injury will be useful for investigation of potential neuroprotective and deleterious effects of targeted microlesions and deep brain stimulation in neurodegenerative diseases.

Key words

Focal brain injury Brain repair Astrocytosis Microgliosis Neurogenesis Cytokines 



Supported in part by VA Merit Grant and Ellis Research Fund.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Neurology and NeurosurgeryUniversity of South FloridaTampaUSA
  2. 2.Department of Neurology, James A. Haley VAH Research ServiceUniversity of South FloridaTampaUSA
  3. 3.College of Medicine NeurologyUniversity of South FloridaTampaUSA
  4. 4.College of Medicine Molecular Pharmacology and PhysiologyUniversity of South FloridaTampaUSA

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