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Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation

  • Cang Chen
  • Michael J. Guderyon
  • Guo Ge
  • Robert A. Clark
  • Senlin LiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)

Abstract

Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.

Key words

Lentiviral vector Co-transfection Viral transduction Bone marrow cells Hematopoietic stem cell transplantation 

Notes

Acknowledgment

The establishment of this method was supported by a Merit Review grant (2I01BX000737) from the Department of Veterans Affairs Biomedical Laboratory Research & Development Program and by the William and Ella Owens Medical Research Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Cang Chen
    • 1
  • Michael J. Guderyon
    • 1
  • Guo Ge
    • 1
  • Robert A. Clark
    • 1
    • 2
  • Senlin Li
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of MedicineUT Health Science Center at San AntonioSan AntonioUSA
  2. 2.Research and Development ServiceAudie L. Murphy VA HospitalSan AntonioUSA
  3. 3.Department of PharmacologyUniversity of Texas Health San AntonioSan AntonioUSA

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