Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach

  • Steven L. Farnsworth
  • Zhifang Qiu
  • Anuja Mishra
  • Peter J. HornsbyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)


The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testing, followed by a modification of the combination and subsequent rounds. While this may produce the desired differentiation in the cell population under study, it is also possible that other strategies may be needed to optimize the differentiation process. These strategies are described in this chapter.

Key words

Pluripotent stem cells Nonhuman primates Differentiation Algorithms Gene expression 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Steven L. Farnsworth
    • 1
  • Zhifang Qiu
    • 1
  • Anuja Mishra
    • 1
  • Peter J. Hornsby
    • 1
    Email author
  1. 1.Department of Cellular and Integrative Physiology and Barshop Institute for Longevity and Aging StudiesUniversity of Texas Health San AntonioSan AntonioUSA

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