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2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

  • Silvia MarinoEmail author
  • Ryan T. Bishop
  • Daniëlle de Ridder
  • Jesus Delgado-Calle
  • Michaela R. Reagan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Key words

In vitro co-culture Osteoclasts Osteoblasts Osteocytes Cancer cells 

Notes

Acknowledgments

We gratefully acknowledge Dr. Aymen I Idris for donating images and his valuable advice and support. S.M. work is supported by the Multiple Myeloma Research Foundation. J.D.C. work is supported by the American Society of Hematology Scholar Award and the International Myeloma Foundation Brian D. Novis Junior Research Grant. M.R.R. the NIH/NIGMS U54GM115516, P30GM106391, P20GM121301, and P30GM103392; the NIH/NIDDK (R24 DK092759-01); the NIH/NIAMS P30AR066261; the American Cancer Society (Research Grant #IRG-16-191-33); and start-up funds from the Maine Medical Center Research Institute.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Silvia Marino
    • 1
    Email author
  • Ryan T. Bishop
    • 2
  • Daniëlle de Ridder
    • 2
  • Jesus Delgado-Calle
    • 3
  • Michaela R. Reagan
    • 4
  1. 1.Division Hematology Oncology, Department of MedicineIndiana University School of MedicineIndianapolisUSA
  2. 2.Department of Oncology and Metabolism, Medical SchoolUniversity of SheffieldSheffieldUK
  3. 3.Department of Anatomy and Cell BiologyIndiana University School of MedicineIndianapolisUSA
  4. 4.Center for Molecular MedicineMaine Medical Centre Research InstituteScarboroughUSA

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