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Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo

  • Janos Kanczler
  • Rahul S. Tare
  • Patrick Stumpf
  • Timothy J. Noble
  • Cameron Black
  • Richard O. C. OreffoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Abstract

In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.

Key words

Stem cells Osteoblast Adipocyte Chondrocyte Bone marrow Diffusion chamber 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Janos Kanczler
    • 1
  • Rahul S. Tare
    • 1
  • Patrick Stumpf
    • 1
  • Timothy J. Noble
    • 1
  • Cameron Black
    • 1
  • Richard O. C. Oreffo
    • 1
    Email author
  1. 1.Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental SciencesUniversity of Southampton, Faculty of MedicineSouthamptonUK

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