Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy
Two-photon microscopy has been widely accepted as a powerful tool to provide both qualitative and quantitative information in bone research. This chapter will describe a step-by-step protocol for using two-photon microscopy to track the colonization of cancer cells to bone using frozen bone samples of xenograft mouse models.
Key wordsTwo-photon microscopy Bone Xenograft Cancer metastasis
- 7.Wang N, Reeves KJ, Brown HK et al (2015) The frequency of osteolytic bone metastasis is determined by conditions of the soil, not the number of seeds; evidence from in vivo models of breast and prostate cancer. J Exp Clin Cancer Res 34:124. https://doi.org/10.1186/s13046-015-0240-8CrossRefPubMedPubMedCentralGoogle Scholar