Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy

  • Gloria Allocca
  • Ning WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


Two-photon microscopy has been widely accepted as a powerful tool to provide both qualitative and quantitative information in bone research. This chapter will describe a step-by-step protocol for using two-photon microscopy to track the colonization of cancer cells to bone using frozen bone samples of xenograft mouse models.

Key words

Two-photon microscopy Bone Xenograft Cancer metastasis 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Oncology and Metabolism, The Mellanby Centre for Bone ResearchThe University of SheffieldSheffieldUK

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