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Transmission Electron Microscopy of Bone

  • Vincent EvertsEmail author
  • Anneke Niehof
  • Wikky Tigchelaar-Gutter
  • Wouter Beertsen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Abstract

Electron microscopic analysis of mineralized tissues like bone and dentin is essential for understanding of cell–cell/cell–matrix interactions, and the three-dimensional organization of these tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources for ultrastructural analysis by transmission electron microscopy.

Key words

Ultrastructure Transmission electron microscopy Bone Mineralized tissue 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vincent Everts
    • 1
    • 2
    Email author
  • Anneke Niehof
    • 2
    • 3
  • Wikky Tigchelaar-Gutter
    • 4
  • Wouter Beertsen
    • 1
    • 2
    • 3
  1. 1.Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of AmsterdamVU University AmsterdamAmsterdamThe Netherlands
  2. 2.Research Institute MOVEVU University AmsterdamAmsterdamThe Netherlands
  3. 3.Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA)University of AmsterdamAmsterdamThe Netherlands
  4. 4.Department of Cell Biology and Histology, Academic Medical Centre (AMC)University of AmsterdamAmsterdamThe Netherlands

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