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Quantitative X-Ray Imaging of Mouse Bone by Faxitron

  • Natalie C. Butterfield
  • John G. Logan
  • Julian Waung
  • Graham R. WilliamsEmail author
  • J. H. Duncan Bassett
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Abstract

This chapter describes the use of point projection digital microradiography for rapid imaging and quantitation of bone mineral content in mice.

Key words

Bone mineral content Digital X-ray analysis Faxitron ImageJ Mouse Subchondral bone Joint disease Skeletal phenotype analysis 

References

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    Waung JA, Maynard SA, Gopal S, Gogakos A, Logan JG, Williams GR, Bassett JHD (2014) Quantitative X-ray microradiography for high-throughput phenotyping of osteoarthritis in mice. Osteoarthr Cartil 22:1396–1400CrossRefGoogle Scholar
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    Glasson SS, Blanchet TJ, Morris EA (2007) The surgical destabilization of the medial meniscus (DMM) model of osteoarthritis in the 129/SvEv mouse. Osteoarthr Cartil 15(9):1061–1069CrossRefGoogle Scholar
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    Demidenko E (2004) Kolmogorov-Smirnov test for image comparison. In: A. Lagana et al. (ed.), Analysis in computer science and its applications, vol 304:6 Springer Berlin, pp 933–939Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Natalie C. Butterfield
    • 1
  • John G. Logan
    • 1
  • Julian Waung
    • 1
  • Graham R. Williams
    • 1
    Email author
  • J. H. Duncan Bassett
    • 1
  1. 1.Molecular Endocrinology Laboratory, Department of MedicineImperial College London, Hammersmith Hospital CampusLondonUK

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