Live Cell Imaging of Bone Cell and Organ Cultures

  • Sarah L. DallasEmail author
  • Patricia A. Veno
  • LeAnn M. Tiede-Lewis
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


Over the past two decades there have been unprecedented advances in the capabilities for live cell imaging using light and confocal microscopy. Together with the discovery of green fluorescent protein and its derivatives and the development of a vast array of fluorescent imaging probes and conjugates, it is now possible to image virtually any intracellular or extracellular protein or structure. Traditional static imaging of fixed bone cells and tissues takes a snapshot view of events at a specific time point, but can often miss the dynamic aspects of the events being investigated. This chapter provides an overview of the application of live cell imaging approaches for the study of bone cells and bone organ cultures. Rather than emphasizing technical aspects of the imaging equipment, which may vary in different laboratories, we focus on what we consider to be the important principles that are of most practical use for an investigator setting up these techniques in their own laboratory. We also provide detailed protocols that our laboratory has used for live imaging of bone cell and organ cultures.

Key words

Live cell imaging Extracellular matrix Osteocytes Bone cells Dynamic imaging 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sarah L. Dallas
    • 1
    Email author
  • Patricia A. Veno
    • 1
  • LeAnn M. Tiede-Lewis
    • 1
  1. 1.Department of Oral and Craniofacial Sciences, School of DentistryUniversity of Missouri, Kansas CityKansas CityUSA

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