Techniques for the Study of Apoptosis in Bone
Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in bone metabolism and associated bone disease or loss of structural integrity. Here we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.
Key wordsApoptosis Osteocyte Bone Caspase Nick translation
- 8.Wong SYP, Evans RA, Needs C, Dunstan C, Hills E, Garvan J (1987) The pathogenesis of osteoarthritis of the hip: evidence for primary osteocyte death. Clin Orthop Relat Res 214:305–312Google Scholar