Techniques for the Study of Apoptosis in Bone

  • Val Mann
  • Brendon Noble
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in bone metabolism and associated bone disease or loss of structural integrity. Here we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.

Key words

Apoptosis Osteocyte Bone Caspase Nick translation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Val Mann
    • 1
  • Brendon Noble
    • 2
  1. 1.Faculty of Medicine and DentistryUniversity of PlymouthPlymouthUK
  2. 2.School of Life Sciences, University of WestminsterLondonUK

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