Isolation and Generation of Osteoblasts

  • Inês P. Perpétuo
  • Lucie E. Bourne
  • Isabel R. OrrissEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.

Key words

Osteoblast Bone formation Mineralization 



The authors gratefully acknowledge the support of The Leverhulme Trust and Arthritis Research UK.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Inês P. Perpétuo
    • 1
  • Lucie E. Bourne
    • 1
  • Isabel R. Orriss
    • 1
    Email author
  1. 1.Department of Comparative Biomedical SciencesRoyal Veterinary CollegeLondonUK

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